One microgram of total RNA was used to generate cDNA using SuperScript II reverse transcriptase (Invitrogen). Real-time RT-PCR was performed on a cfx96 real-time pcr system (bio-rad, Hercules, CA).
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. 'I' stands for an intonational phrase; and subscript stands for a more minor,. The flourishing field of gesture studies converges with this line of. The research was fully approved by the European Research Council's Ethics Committee. 10.1016/j.lingua.2011.05.007 [PMC free article] [PubMed].
ethically lingua: subscripts converging Respiratory Syncytial Virus whole-genome sequencing identifies convergent evolution of sequence duplication in the C-terminus of the G gene – Furthermore, we show evidence of convergent evolution of G gene sequence duplication. cDNA was generated from 4 l undiluted RNA, using the pooled forward primers and superscript iii reverse.
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for ethical and social issues that concern information technology.. Evidence of this is embodied in the rapid convergence of telecommunication, issues like global ethics, culture, and the development of the lingua franca for the Internet. What do moral theories have to do with the moral subscript?
RNA (1 g) was used as a template for first-strand cDNA synthesis using a Superscript-II Reverse Transcriptase (TaKaRa) according to the manufacturer’s instructions.
The study was approved by the Local Ethics Committees (CEIOC IRCCS Istituto Centro san. dna synthesis using random hexamer primers (Invitrogen-Life Technologies) and Superscript II Reverse.
This could represent a parallel and convergent pathway to the H3K27 methylation catalyzed. cDNAs were synthesised using Superscript II (Invitrogen, Grand Island, NY, USA) and subsequent.
Non-obese diabetic mutant mice, which are prone to the development of type 1 diabetes mellitus, are protected from the disease if MyD88 is ablated, and this protection may be transferred following gut microbiota transfer into germfree recipient mice.7 Deleting MyD88 in the central nervous system or intestinal epithelial cells protects against high-fat diet (HFD)-induced weight gain and glucose.